Professional ethics is one of the most important topics in medicine that educators or professors play an important role in teaching and measuring it. The present study was designed and conducted to evaluate the observance of professional ethics among surgical assistants of educational hospitals of Ahvaz Jundishapur University of Medical Sciences in 2019 by self-assessment method. This study was a cross-sectional study. Each of the assistants completed a standard questionnaire that included the variables required for the study. The sample was selected according to population of surgical assistants in the general and orthopedic departments, who entered the study using census sampling method and were equal to 47 people. Of 47 participants, 29 were assistant general surgeons and 18 were orthopedic surgeons. Observance of professional ethics was acceptable. There was a significant relationship between knowledge and communication skills and assistants in both general and orthopedic departments (P <0.05). There was a significant relationship between professional ethics and assistants in both general and orthopedic departments (P<0.05). Respect for others with a mean of 1.66±6.0, had the highest mean rate. Also, interpersonal and social skills with an average of 2.17±4.48, had the lowest mean. Considering that inclusion of professional ethics in laws and regulations is one of the important and vital factors in growth and development of ethics, institutionalization of professional ethics in strategic plan should be considered by policy makers and structure, training and evaluation of professional ethics be reviewed.