One of the most effective factors on organizational behavior is the Nurses’ job satisfaction. Spiritual wellbeing of nurses is an inseparable personal element that influences their job satisfaction. The aim of this study was assessing association between Nurses' spiritual well-being and job satisfaction in Educational-Therapeutic center of Tabriz Shohada. The study population was nurses of Shohada hospital. In this study, 155 participants were selected by stratified Simple random sampling method. The research tools consisted of two questionnaires including spiritual well-being scale (SWBS) and job satisfaction (MSQ). In order to analyze the data, descriptive (frequency, percent, mean, standard deviation) and inferential (independent t, Pearson, Spearman, ANOVA Tukey test) were used. The results showed that there is a positive and significant relationship between nurses' spiritual well-being and their job satisfaction (P<0.05). Also, mean score of nurses' job satisfaction was 61.89 (12.63) and mean score of spiritual well-being was 89.85 (13.64) and both were above average (P<0.05). Type of employment, age, and sex had significant relationship with job satisfaction (P<0.05). According to the findings of this study, it is recommended that the relevant stakeholders and authorities increase their nurses' job satisfaction, pay special attention to the issue of spiritual health and improvement of it, because increasing spiritual health increases nurses’ job satisfaction and ultimately lead to better Care services will be better for patients.